Application
Western blot analysis of PKC γ expression in non-transfected: (A) and human PKC γ transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.Application
PKC antibodies used include PKC α (C-20), PKC βI (C-16), PKC βII (C-18), PKC γ (C-19), PKC ε (C-15), PKC ζ (C-20) and PKC η (C-15).Application
Immunofluorescence staining of methanol-fixed 3611-RF cells showing cytoplasmic staining.Application
Western blot analysis of PKC γ expression in 3611-RF transformed rat fibroblasts.| Product Name | PKC γ Antibody (C-19) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of PKC γ of mouse origin |
| Modification Notes | Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into many different isoforms (å, ∫I, ∫II, ©, ∂, é, Ω, h, †, λ/î, µ and n). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC ∂ and é are independent of Ca++. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PKC γ of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of PKC &gamma of mouse origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of PKC γ of mouse origin |
| Entrez Gene | 5582 |
|---|
Application
Western blot analysis of PKC γ expression in non-transfected: (A) and human PKC γ transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.
Application
PKC antibodies used include PKC α (C-20), PKC βI (C-16), PKC βII (C-18), PKC γ (C-19), PKC ε (C-15), PKC ζ (C-20) and PKC η (C-15).
Application
Immunofluorescence staining of methanol-fixed 3611-RF cells showing cytoplasmic staining.
Application
Western blot analysis of PKC γ expression in 3611-RF transformed rat fibroblasts.| Application Notes | recommended for detection of PKC γ of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
