Application
Western blot analysis of CGBP expression in K-562 whole cell lysate.Application
Western blot analysis of CGBP expression in non-transfected: (A) and human CGBP transfected: (B) 293 whole cell lysates and Jurkat nuclear extract (C).| Product Name | CGBP Antibody (H-120) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 61-180 of CGBP of human origin |
| Modification Notes | The human genome contains discrete clusters of unmethylated CpG dinucleotides, called CpG islands, which contribute to the modulation of gene expression by binding transcription factors (1-4). Human CpG binding protein (CGBP), detected in K562 cells, is a widely expressed member of CpG binding proteins that requires the CpG dinucleotide to bind DNA (1). CGBP binds specifically to unmethylated CpG motifs and functions as a transcriptional activator (1). The CXXC domain of CGBP is conserved in DNA methyltransferase, human trithorax, and methyl-CpG binding protein (MBP), and is involved in DNA-binding (1,5,6). CGBP also contains several domains implicated in protein-protein interactions, such as a coiled-coil domain, and two PHD finger domains, the function of which remains to be determined (1). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of CGBP of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 61-180 of CGBP of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 61-180 of CGBP of human origin |
| Entrez Gene | 30827 |
|---|
Application
Western blot analysis of CGBP expression in K-562 whole cell lysate.
Application
Western blot analysis of CGBP expression in non-transfected: (A) and human CGBP transfected: (B) 293 whole cell lysates and Jurkat nuclear extract (C).| Application Notes | recommended for detection of CGBP of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
