Application
Western blot analysis of LIMP II expression in Y79 whole cell lysate.Application
Western blot analysis of LIMP II expression in non-transfected: (A) and human LIMP II transfected: (B) 293 whole cell lysates.Application
Western blot analysis of LIMP II expression in HeLa whole cell lysate.Application
Western blot analysis of LIMP II expression in non-transfected: (A) and human LIMP II transfected: (B) whole cell lysates.| Product Name | LIMP II Antibody (H-230) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 249-478 mapping at the C-terminus of LIMP II of human origin |
| Modification Notes | Lysosomes are intracytoplasmic organelles that are found within leukocytes (granulocytes, neutrophils, basophils and eosinophils) and function as storage granules for small particles. Lysosomes actively support subcellular protein degradation mechanisms through fusion with cellular organelles such as phagocytic vacuoles and the plasma membrane. Lysosome fusion to the plasma membrane, known as exocytosis, releases the contents of the vesicle into the extracellular environment. The lysosomal integral membrane proteins I–III, known as LIMP-I, LIMP-II and LIMP-III, localize from the trans-Golgi network to lysosomes via an AP-3-dependent pathway that may involve AP-1 and Clathrin. LIMP I-III are protein markers for the lysosome organelle. These markers are exceptionally useful for microscopy studies, cellular fractionation validation and studies pertaining to protein trafficking through the secretory pathway. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of LIMP II of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 249-478 mapping at the C-terminus of LIMP II of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 249-478 mapping at the C-terminus of LIMP II of human origin |
| Entrez Gene | 950 |
|---|
Application
Western blot analysis of LIMP II expression in Y79 whole cell lysate.
Application
Western blot analysis of LIMP II expression in non-transfected: (A) and human LIMP II transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of LIMP II expression in HeLa whole cell lysate.
Application
Western blot analysis of LIMP II expression in non-transfected: (A) and human LIMP II transfected: (B) whole cell lysates.| Application Notes | recommended for detection of LIMP II of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
