Application
Western blot analysis of NFATc2 phosphorylation in Ramos whole cell lysate.Application
Western blot analysis of NFATc2 phosphorylation in untreated (A) and lambda protein phosphatase treated (B) MOLT-4 whole cell lysates.Application
Western blot analysis of NFATc2 phosphorylation in untreated (A) and lambda protein phosphatase treated (B) Jurkat whole cell lysates.Application
Western blot analysis of NFATc2 phosphorylation in untreated (A,C) and IL-2-treated (B,D) Jurkat whole cell lysates. Antibody was preincubated with cognate phosphorylated peptide in blots (C) and (D).| Product Name | p-NFATc2 Antibody (Ser 326) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Ser 326 of NFATc2 of human origin |
| Modification | Phosphoralated |
| Modification Notes | The NFAT (nuclear factor of activated T cells) family of transcription factors regulates cytokine expression in T cells. Members of the family include NFATc1 (NFATc), NFATc2 (NFATp), NFATn, NFATc3 (NFAT4, NFATx) and NFATc4 (NFAT3). Recognition of antigen by the T cell receptor (TCR) eventually activates the calcium-dependent protein phosphatase calcineurin. Once activated, calcineurin stimulates the translocation of NFATc1 (cytoplasmic) from the cytoplasm to the nucleus where it associates with NFATn (nuclear). Like NFATc1, NFATc2 resides in the cytoplasm and translocates to the nucleus subsequent to activation of calcineurin. Once in the nucleus, NFATc2 synergizes with AP-1 transcription factors to initiate transcription of cytokine genes. NFATc3 and NFATc4 share 65% sequence identity with other members of the NFAT family. They are similar to NFATc2 in that they also synergize with the AP-1 family of proteins. NFATc2 is phosphorylated by NFATC-kinase and is inducibly expressed in T lymphocytes after TCR complex activation. The phosphorylated form of NFATc2 localizes to the cytoplasm. It is dephosphorylated by calcineurin and localizes to the nucleus after dephosphorylation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Ser 326 phosphorylated NFATc2 KTS domain of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 326 of NFATc2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 326 of NFATc2 of human origin |
| Entrez Gene | 4773 |
|---|
Application
Western blot analysis of NFATc2 phosphorylation in Ramos whole cell lysate.
Application
Western blot analysis of NFATc2 phosphorylation in untreated (A) and lambda protein phosphatase treated (B) MOLT-4 whole cell lysates.
Application
Western blot analysis of NFATc2 phosphorylation in untreated (A) and lambda protein phosphatase treated (B) Jurkat whole cell lysates.
Application
Western blot analysis of NFATc2 phosphorylation in untreated (A,C) and IL-2-treated (B,D) Jurkat whole cell lysates. Antibody was preincubated with cognate phosphorylated peptide in blots (C) and (D).| Application Notes | recommended for detection of Ser 326 phosphorylated NFATc2 KTS domain of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
