Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human epididymis tissue showing nuclear and cytoplasmic staining of glandular cells.Application
Western blot analysis of AR expression in NIH/3T3 whole cell lysate.Application
Western blot analysis of AR expression in T-47D (A), ZR-75-1 (B), MCF7 (C), LNCaP (D) and AT-3 (E) whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human prostate carcinoma tissue showing nuclear staining of glandular epithelia (A). Immunofluorescence staining of methanol-fixed LNCaP cells showing nuclear localization (B).Application
Antibodies tested include AR (N-20): (A-E) and AR (441): (F).| Product Name | AR Antibody (N-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the N-terminus of AR of human origin |
| Modification Notes | Androgens exhibit a wide range of effects on the development, maintenance and regulation of male phenotype and male reproductive physiology. The androgen receptor (AR) is a member of the steroid superfamily of ligand-dependent transcription factors. ARs bind the two biologically active androgens, testosterone (T) and dihydrotestosterone (DHT), with high and nearly identical affinities; however, the rates of association and dissociation of T are about three times more rapid than those of DHT. This difference has resulted in speculation as to whether these differences in binding kinetics could account for the different physiological effects of T and DHT. A striking feature of AR is its rapid degradation in the absence of ligand. It is now well established that androgen binding results in an at least six-fold increase in androgen stability and that ligand-induced stabilization of AR is highly androgen- specific. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of androgen receptor of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the N-terminus of AR of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the N-terminus of AR of human origin |
| Entrez Gene | 367 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human epididymis tissue showing nuclear and cytoplasmic staining of glandular cells.
Application
Western blot analysis of AR expression in NIH/3T3 whole cell lysate.
Application
Western blot analysis of AR expression in T-47D (A), ZR-75-1 (B), MCF7 (C), LNCaP (D) and AT-3 (E) whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human prostate carcinoma tissue showing nuclear staining of glandular epithelia (A). Immunofluorescence staining of methanol-fixed LNCaP cells showing nuclear localization (B).
Application
Antibodies tested include AR (N-20): (A-E) and AR (441): (F).| Application Notes | recommended for detection of androgen receptor of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
