Application
Immunofluorescence staining of methanol-fixed SK-MEL-28 cells showing cytoplasmic localization.Application
Western blot analysis of RICK expression in SK-MEL-28 whole cell lysate.| Product Name | RICK Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 241-540 of RICK of human origin |
| Modification Notes | Members of the tumor necrosis factor receptor (TNFR) family play a key role in the induction of NFkB activation and cell death. These receptors recruit and assemble signaling complexes that contain a number of death-domain containing proteins, such as RIP. RICK, also designated RIP2 and CARDIAK, is a RIP-like protein kinase involved in regulating both TNFR and CD95-mediated apoptosis. RICK contains an N-terminal serine-threonine kinase catalytic domain and a C-terminal caspase-recruiting domain. The C-terminal domain is sufficient for the apoptotic functions of the protein, while the whole protein is required for the activation of NFkB. RICK binds specifically to a number of proteins in the TNFR-associated factor (TRA≠F) family, and these TRAF interactions are involved in recruiting RICK to receptor signaling complexes. Overexpression of RICK leads to the activation of caspase-8 and potentiates apoptosis induced by Fas ligand, FADD, CLARP and caspase-8. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of RICK of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 241-540 of RICK of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 241-540 of RICK of human origin |
| Entrez Gene | 8767 |
|---|
Application
Immunofluorescence staining of methanol-fixed SK-MEL-28 cells showing cytoplasmic localization.
Application
Western blot analysis of RICK expression in SK-MEL-28 whole cell lysate.| Application Notes | recommended for detection of RICK of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
