Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing nuclear staining of neuronal and glial cells.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human malignant glyoma tissue showing nuclear staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse brain tissue showing nuclear localization.Application
Western blot analysis of Arnt 2 expression in WEHI-231 (A) whole cell lysate and mouse brain (B) extract.| Product Name | Arnt 2 Antibody (M-165) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 457-622 of Arnt 2 of mouse origin |
| Modification Notes | AhR, Arnt 1, Arnt 2 and BMAL1 are members of a family of transcription factors that contain a basic helix-loop-helix motif and a common “PAS” motif. The aromatic (aryl) hydrocarbon receptor, AhR, is a ligand dependent transcription factor that interacts with specific DNA sequences termed xenobiotic responsive elements (XREs) to activate several genes including CYP1A1, glutathione S-transferase Ya subunit and DT-diaphorase. The Ah receptor nuclear translocator proteins (Arnt 1 or Arnt 2) are required for ligand-dependent nuclear translocation of the Ah receptor and are also necessary for Ah receptor binding to the XRE element. Arnt 2 (aryl hydrocarbon receptor nuclear translocator 2), also known as Hif-2b or bHLHe1, is a 712 amino acid nuclear protein that is exclusively expressed in adult brain and kidney. Containing a basic helix-loop-helix (bHLH) domain, a PAC (PAS-associated C-terminal) domain and two PAS (PER-ARNT-SIM) domains, Arnt 2 specifically recognizes the xenobiotic response element (XRE). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of Arnt 2 of mouse, rat, human and zebrafish origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 457-622 of Arnt 2 of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 457-622 of Arnt 2 of mouse origin |
| Entrez Gene | 9915 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing nuclear staining of neuronal and glial cells.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human malignant glyoma tissue showing nuclear staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse brain tissue showing nuclear localization.
Application
Western blot analysis of Arnt 2 expression in WEHI-231 (A) whole cell lysate and mouse brain (B) extract.| Application Notes | recommended for detection of Arnt 2 of mouse, rat, human and zebrafish origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
