Application
Western blot analysis of CstF-64 expression in HeLa (A), Jurkat (B) and K-562 (C) nuclear extracts.Application
Antibodies tested include p-CstF-64 (Ser 83)-R: (A,B,C) and CstF-64 (H-300): (D,E,F).Application
Western blot analysis of CstF-64 expression in non-transfected 293T: (A), human CstF-64 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of CstF-64 expression in non-transfected: (A) and human CstF-64 transfected: (B) 293T whole cell lysates and BJAB nuclear extract (C).Application
Western blot analysis of CstF-64 expression in 293T (A) and HeLa (B) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of CstF-64 expression in BJAB (A) and THP-1 (B) nuclear extracts.| Product Name | CstF-64 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 278-577 mapping at the C-terminus of CstF-64 of human origin |
| Modification Notes | Polyadenylation of mRNA precursors is a two-step reaction that requires multiple protein factors. The first step, endonucleolytic cleavage of polyadenylation substrates, requires CstF (cleavage stimulation factor), a heterotrimer that is composed of three distinct subunits. CstF-64 contains an RNA binding domain and is responsible for the RNA binding activity of CstF. CstF-64 is expressed in all somatic cells and in pre- and postmeiotic, but not meiotic, germ cells. However, a large variant of CstF-64, called t CstF-64, is abundantly expressed in meiotic and postmeiotic cells in the testis and to a lesser extent in the brain, and promotes the germ cell pattern of polyadenylation. The gene encoding CstF-64 (designated CSTF2) maps to the X chromosome, whereas t CstF-64 is encoded by an autosomal gene. The increase in CstF-64 concentration during B cell activation switches IgM heavy chain mRNA expression from membrane-bound to secreted forms, suggesting that CstF-64 plays a key role in regulating IgM heavy chain expression during B cell differentiation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CstF-64 and its tau variant of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 278-577 mapping at the C-terminus of CstF-64 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 278-577 mapping at the C-terminus of CstF-64 of human origin |
| Entrez Gene | 1478 |
|---|
Application
Western blot analysis of CstF-64 expression in HeLa (A), Jurkat (B) and K-562 (C) nuclear extracts.
Application
Antibodies tested include p-CstF-64 (Ser 83)-R: (A,B,C) and CstF-64 (H-300): (D,E,F).
Application
Western blot analysis of CstF-64 expression in non-transfected 293T: (A), human CstF-64 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of CstF-64 expression in non-transfected: (A) and human CstF-64 transfected: (B) 293T whole cell lysates and BJAB nuclear extract (C).
Application
Western blot analysis of CstF-64 expression in 293T (A) and HeLa (B) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of CstF-64 expression in BJAB (A) and THP-1 (B) nuclear extracts.| Application Notes | recommended for detection of CstF-64 and its tau variant of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
