Application
Western blot analysis of USF-2 expression in non-transfected 293T: (A), mouse USF-2 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of USF-2 expression in non-transfected: (A) and mouse USF-2 transfected: (B) 293T whole cell lysates and Jurkat nuclear extract (C).Application
Western blot analysis of USF-2 expression in non-transfected control (A) and USF-2 siRNA transfected (B) HeLa cells. Blot probed with USF-2 (N-18): . GAPDH (FL-335): used as specificity and loading control.Application
Western blot analysis of USF-2 p44 expression in HeLa (A), Jurkat (B), K-562 (C), A-431 (D) and phorbol-induced NIH/3T3 (E) nuclear extracts.| Product Name | USF-2 Antibody (N-18) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the N-terminus of USF-2 of mouse origin |
| Modification Notes | The ubiquitously expressed cellular upstream stimulatory factor (USF) consists of USF-1 and USF-2 polypeptides which independently exhibit site-specific DNA binding and are members of the c-Myc-related family of regulatory factors containing helix-loop-helix domains. USF also contains a leucine repeat that is required for efficient DNA binding. USF was originally identified as an upstream stimulatory factor that binds the core sequence CACGTG in the adenovirus late promoter. These findings, together with the demonstration of cooperative interaction between USF and the initiator-binding protein, TFII-I , raises the possibility of a more general involvement of USF in transcriptional regulation. While expression of both USF-1 and USF-2 species is ubiquitous, different ratios of USF homo- and heterodimers are found in different cell types. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ChIPELISAGSIFIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.: WB:1:100~1:200 IF:1:20~1:100 ELISA:1:1000~5000 IP:1:20~1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the N-terminus of USF-2 of mouse origin |
|---|---|
| Tissue Specificity | recommended for detection of USF-2 p44 of mouse, rat and human origin by WB, IP, IF and ELISA |
| Entrez Gene | 7392 |
|---|
Application
Western blot analysis of USF-2 expression in non-transfected 293T: (A), mouse USF-2 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of USF-2 expression in non-transfected: (A) and mouse USF-2 transfected: (B) 293T whole cell lysates and Jurkat nuclear extract (C).
Application
Western blot analysis of USF-2 expression in non-transfected control (A) and USF-2 siRNA transfected (B) HeLa cells. Blot probed with USF-2 (N-18): . GAPDH (FL-335): used as specificity and loading control.
Application
Western blot analysis of USF-2 p44 expression in HeLa (A), Jurkat (B), K-562 (C), A-431 (D) and phorbol-induced NIH/3T3 (E) nuclear extracts.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.: WB:1:100~1:200 IF:1:20~1:100 ELISA:1:1000~5000 IP:1:20~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
