Application
Western blot analysis of Jun B expression in non-transfected: (A) and human Jun B transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Jun B expression in NIH/3T3 nuclear extract.Application
Western blot analysis of Jun B expression in HeLa whole cell lysate.Application
Western blot analysis of Jun B expression in non-transfected: (A) and mouse Jun B transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Jun B expression in phorbol ester-induced NIH/3T3 (A), untreated KNRK (B) and phorbol ester-induced KNRK (C) nuclear extracts.| Product Name | Jun B Antibody (210) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping near the C-terminus of Jun B of mouse origin |
| Modification Notes | The c-Jun proto-oncogene was first identified as the cellular homolog of the avian sarcoma virus v-Jun oncogene. The c-Jun protein, along with c-Fos, is a component of the AP-1 transcriptional complex. c-Jun can form either Jun/Jun homodimers or Jun/Fos heterodimers via the leucine repeats in both proteins. Homo- and heterodimers bind to the TGACTCA consensus sequence present in numerous promoters and initially identified as the phorbol ester tumor promoter response element (TRE). Two additional genes, Jun B andJun D, have been shown to be almost identical to c-Jun in their C-terminal regions, which are involved in dimerization and DNA binding, whereas their N-terminal domains, which are involved in transcriptional activation, diverge. All three form heterodimers among themselves and with c-Fos and other members of the Fos gene family. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of Jun B of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the C-terminus of Jun B of mouse origin |
|---|---|
| Tissue Specificity | epitope mapping near the C-terminus of Jun B of mouse origin |
| Entrez Gene | 3726 |
|---|
Application
Western blot analysis of Jun B expression in non-transfected: (A) and human Jun B transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Jun B expression in NIH/3T3 nuclear extract.
Application
Western blot analysis of Jun B expression in HeLa whole cell lysate.
Application
Western blot analysis of Jun B expression in non-transfected: (A) and mouse Jun B transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Jun B expression in phorbol ester-induced NIH/3T3 (A), untreated KNRK (B) and phorbol ester-induced KNRK (C) nuclear extracts.| Application Notes | recommended for detection of Jun B of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
