Application
Antibodies tested include p-Rac 1 (Ser 71)-R: (A,B) and Rac 1 (C-11): (C,D).Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of Rac 1 expression in HL-60 whole cell lysate.Application
Western blot analysis of Rac 1 expression in A-431 whole cell lysate.Application
Western blot analysis of Rac 1 expression in non-transfected 293T: (A), mouse Rac 1 transfected 293T: (B) and A-431 (C) whole cell lysates.Application
Western blot analysis of Rac 1 expression in non-transfected: (A) and human Rac 1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Rac 1 expression in non-transfected: (A) and mouse Rac 1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of human recombinant Rac 1 fusion protein.| Product Name | Rac 1 Antibody (C-11) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | A large number of low molecular weight, GTP binding proteins of the Ras superfamily have been identified. These proteins regulate many fundamental processes in all eukaryotic cells such as growth, vesicle traffic and cytoskeletal organization. GTPase-activating proteins (GAPs) accelerate the intrinsic rate of GTP hydrolysis of Ras-related proteins, resulting in downregulation of their active form. Two proteins in this family, Rac 1 and Rac 2, are 92% identical and share GTP binding and GTP hydrolysis motifs with other members of the Ras superfamily. Rac 1 is expressed in a large number of different cell types. Rac 2 is primarily expressed only in myeloid cells and has been reported to be a regulatory component of the human neutrophil NADPH oxidase. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Rac 1 and, to a lesser extent, Rac 3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA non cross-reactive with Rac 2 also reactive with additional species, including canine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within the C-terminus of Rac 1 of human origin |
|---|---|
| Tissue Specificity | epitope mapping within the C-terminus of Rac 1 of human origin |
| Entrez Gene | 5879 |
|---|
Application
Antibodies tested include p-Rac 1 (Ser 71)-R: (A,B) and Rac 1 (C-11): (C,D).
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of Rac 1 expression in HL-60 whole cell lysate.
Application
Western blot analysis of Rac 1 expression in A-431 whole cell lysate.
Application
Western blot analysis of Rac 1 expression in non-transfected 293T: (A), mouse Rac 1 transfected 293T: (B) and A-431 (C) whole cell lysates.
Application
Western blot analysis of Rac 1 expression in non-transfected: (A) and human Rac 1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Rac 1 expression in non-transfected: (A) and mouse Rac 1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of human recombinant Rac 1 fusion protein.| Application Notes | recommended for detection of Rac 1 and, to a lesser extent, Rac 3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA non cross-reactive with Rac 2 also reactive with additional species, including canine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
