Application
Western blot analysis of USF-2 expression in HeLa (A), NIH/3T3 (B), A-431 (C), Jurkat (D), K-562 (E) and IMR-32 (F) nuclear extracts.Application
Western blot analysis of USF-2 expression in non-transfected 293T: (A), mouse USF-2 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of USF-2 expression in non-transfected: (A) and mouse USF-2 transfected: (B) 293T whole cell lysates and Jurkat nuclear extract (C).Application
Immunofluorescence staining of methanol-fixed, control HeLa (A) and USF-2 siRNA silenced HeLa (B) cells showing diminished nuclear staining in the siRNA silenced cells. Cells probed with USF-2 (C-20): .Application
Western blot analysis of USF-2 p44 expression in NIH/3T3 (A) and KNRK (B) nuclear extracts.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing nuclear staining.Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing nuclear staining.| Product Name | USF-2 Antibody (C-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The ubiquitously expressed cellular upstream stimulatory factor (USF) consists of USF-1 and USF-2 polypeptides which independently exhibit site-specific DNA binding and are members of the c-Myc-related family of regulatory factors containing helix-loop-helix domains. USF also contains a leucine repeat that is required for efficient DNA binding. USF was originally identified as an upstream stimulatory factor that binds the core sequence CACGTG in the adenovirus late promoter. These findings, together with the demonstration of cooperative interaction between USF and the initiator-binding protein, TFII-I , raises the possibility of a more general involvement of USF in transcriptional regulation. While expression of both USF-1 and USF-2 species is ubiquitous, different ratios of USF homo- and heterodimers are found in different cell types. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of USF-2 p44 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of USF-2 of mouse origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of USF-2 of mouse origin |
| Entrez Gene | 7392 |
|---|
Application
Western blot analysis of USF-2 expression in HeLa (A), NIH/3T3 (B), A-431 (C), Jurkat (D), K-562 (E) and IMR-32 (F) nuclear extracts.
Application
Western blot analysis of USF-2 expression in non-transfected 293T: (A), mouse USF-2 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of USF-2 expression in non-transfected: (A) and mouse USF-2 transfected: (B) 293T whole cell lysates and Jurkat nuclear extract (C).
Application
Immunofluorescence staining of methanol-fixed, control HeLa (A) and USF-2 siRNA silenced HeLa (B) cells showing diminished nuclear staining in the siRNA silenced cells. Cells probed with USF-2 (C-20): .
Application
Western blot analysis of USF-2 p44 expression in NIH/3T3 (A) and KNRK (B) nuclear extracts.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing nuclear staining.
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing nuclear staining.| Application Notes | recommended for detection of USF-2 p44 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
