Application
Western blot analysis of APC5 expression in non-transfected: (A) and mouse APC5 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of APC5 expression in non-transfected 293T: (A), mouse APC5 transfected 293T: (B) and Ramos (C) whole cell lysates.Application
Western blot analysis of APC5 expression in A-431 whole cell lysate.| Product Name | APC5 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The anaphase-promoting complex (APC) is composed of more than ten subunits, including APC1, APC2, APC4, APC5, APC7, APC8, APC10 and APC11. The APC acts in a cell-cycle dependent manner to promote the separation of sister chromatids during the transition between metaphase and anaphase in mitosis. APC, or cyclosome, accomplishes this progression through the ubiquitination of mitotic cyclins and other regulatory proteins that are targeted for destruction during cell division. APC is phosphorylated, and thus activated, by protein kinases Cdk1/cyclin B and polo-like kinase (Plk). APC is under tight control by a number of regulatory factors, including CDC20, CDH1 and MAD2. Specifically, CDC20 and CDH1 directly bind to and activate the cyclin-ubiquitination activity of APCs. In contrast, MAD2 inhibits APC by forming a ternary complex with CDC20 and APC, thus preventing APC activation. APC5, also known as ANAPC5 or PDL-108, is a subunit of APC that mediates the interaction of APC with the transcription coactivators CBP and p300. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of APC5 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of APC5 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of APC5 of human origin |
| Entrez Gene | 51433 |
|---|
Application
Western blot analysis of APC5 expression in non-transfected: (A) and mouse APC5 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of APC5 expression in non-transfected 293T: (A), mouse APC5 transfected 293T: (B) and Ramos (C) whole cell lysates.
Application
Western blot analysis of APC5 expression in A-431 whole cell lysate.| Application Notes | recommended for detection of APC5 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
