Application
Western blot analysis of p19 expression in non-transfected: (A) and mouse p19 transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.Application
Western blot analysis of mouse recombinant p19 fusion protein.| Product Name | p19 Antibody (M-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The normal progression of cells through the cell cycle is under the control of the cyclin dependent protein kinases Cdk4 and Cdk6, which are subject to inhibition by the mitotic inhibitory protein, p16. Isolated members of the p16 family have been designated p15, p18 and p19. p15 expression is upregulated approximately 30-fold in TGF ß-treated human keratinocytes, suggesting that p15 may function as an effector of TGF ß-mediated cell cycle arrest through inhibition of Cdk4 and Cdk6 kinases. The gene encoding p15 has been mapped to chromosome 9p21.3 at a position adjacent to the p16 gene, at a site of frequent chromosomal abnormality in human tumors. Two p16- related proteins, p19 and p18, specifically inhibit the kinase activities of Cdk4 and Cdk6 but do not affect those of cyclin E-Cdk2, cyclin A-Cdk2 or cyclin B-Cdc2 complexes. p19 is expressed at maximal level during S phase, while overexpression of p19 leads to G1 arrest. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of p19 of mouse and rat origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of p19 of mouse origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of p19 of mouse origin |
| Entrez Gene | 12581 |
|---|
Application
Western blot analysis of p19 expression in non-transfected: (A) and mouse p19 transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.
Application
Western blot analysis of mouse recombinant p19 fusion protein.| Application Notes | recommended for detection of p19 of mouse and rat origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
