Application
Immunofluorescence staining of methanol-fixed Wi38 cells showing nuclear and cytoplasmic localization.Application
Western blot analysis of A20 expression in uninduced MCF7 (A) and TNFα induced MCF7 (B) whole cell lysates.WB
Differences in AMPKα activation levels and Myosin10 protein levels, *: Compared with the control group, p < 0.05.WB
Co-localization and interaction of p-AMPK and Myosin10WB
The effects of Metformin and ML-7 treatment on the invasion and migration of KMH-2 and TCP-1 cells; Control represents the control group, Metformin represents the metformin treatment group. A: Cell migration condition; B: Cell invasion condition (Compared with the control group, *p < 0.05).| Product Name | A20 Antibody (H-100) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | A20 is a Cys2/Cys2 zinc finger protein that is induced by a variety of inflammatory stimuli and regulates gene expression. Specifically, A20 is induced by tumor necrosis factor (TNF) and interleukin 1 (IL-1), and acts as a negative regulator of nuclear factor κ B (NFκB) gene expression. By inhibiting NFκB activation, A20 plays a critical role in terminating NFκB responses to various stimuli. Although the C-terminal region of A20 contains seven zinc finger domains, only four of these domains are required for in vitro inhibition of TNF-induced NFκB activation. A20 also interacts with several other proteins, such as TRAF2, TRAF6 and IκB kinase (IKK) γ protein, and can thereby inhibit cell death. TXBP151, a novel A20-binding protein, may mediate the anti-apoptotic activity of A20. Involved in the negative feedback regulation of signal transduction, A20 and A20-binding proteins may be useful as novel therapeutic tools in the treatment of a variety of diseases. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ChIPELISAGSIFIPWB |
| recommended for detection of A20 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-100 mapping at the N-terminus of A20 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-100 mapping at the N-terminus of A20 of human origin |
| Entrez Gene | 7128 |
|---|
Application
Immunofluorescence staining of methanol-fixed Wi38 cells showing nuclear and cytoplasmic localization.
Application
Western blot analysis of A20 expression in uninduced MCF7 (A) and TNFα induced MCF7 (B) whole cell lysates.
WB
Differences in AMPKα activation levels and Myosin10 protein levels, *: Compared with the control group, p < 0.05.
WB
Co-localization and interaction of p-AMPK and Myosin10
WB
The effects of Metformin and ML-7 treatment on the invasion and migration of KMH-2 and TCP-1 cells; Control represents the control group, Metformin represents the metformin treatment group. A: Cell migration condition; B: Cell invasion condition (Compared with the control group, *p < 0.05).| Application Notes | recommended for detection of A20 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
