Application
Western blot analysis of TACC3 expression in non-transfected 293T: (A), mouse TACC3 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of TACC3 expression in non-transfected 293T: (A), mouse TACC3 transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of TACC3 expression in non-transfected 293T: (A), human TACC3 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of TACC3 expression in HeLa whole cell lysate.| Product Name | TACC3 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 of TACC3 of human origin |
| Modification Notes | TACC1 (transforming acidic coiled coil gene 1) is one of three TACC family members, which are thought to be involved in breast tumorigenesis. TACC1 is located on 8p11, in a chromosomal region that is amplified in approximately 15% of all breast tumor samples. The short arm of chromosome 8 also contains FGFR1, whose expression is enhanced in most breast cancer tumors. TACC family members TACC1, TACC2, and TACC3 map very closely to the corresponding FGFR1, FGFR2, FGFR3 genes on chromosomes 8, 10, and 4. Subsequently, since they are phylogenetically related, it is proposed that TACC and FGFR have similar roles in cell growth and differentiation. Also, TACC1 contains a conserved C-terminal region as in the Drosophila homolog D-TACC. D-TACC has been shown to be necessary for normal spindle function, and the mammalian TACC proteins appears to interact with centrosomes and microtubules in a similar manner. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of TACC3 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 of TACC3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 of TACC3 of human origin |
| Entrez Gene | 10460 |
|---|
Application
Western blot analysis of TACC3 expression in non-transfected 293T: (A), mouse TACC3 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of TACC3 expression in non-transfected 293T: (A), mouse TACC3 transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of TACC3 expression in non-transfected 293T: (A), human TACC3 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of TACC3 expression in HeLa whole cell lysate.| Application Notes | recommended for detection of TACC3 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
