Application
Western blot analysis of human recombinant MLH3 fusion protein.| Product Name | MLH3 Antibody (H-226) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1228-1453 mapping at the C-terminus of MLH3 of human origin |
| Modification Notes | DNA mismatch repair (MMR) is essential for maintaining the integrity of the genome during replication. This process is highly conserved across bacterial and eukaryotic systems as many of the genes expressed in bacteria are closely related to the yeast and mammalian homologs. In bacteria two proteins, MutS and MutL, form homodimeric complexes that are responsible for recognizing and facilitating MMR. Human homologs of these proteins include MSH2 and MSH3 (MutS homolog 2 and 3), and the corresponding human homologs of MutL are hMLH1, PMS1, PMS2 and MLH3. MSH2 and MSH3 form heterodimers that cooperatively mediate MMR. MLH3 preferentially dimerizes with MLH1 to repair DNA mismatches and restore the stability to the genome. Mutations in the genes encoding MSH2 and MLH1 induce microsatellite instablitiy of the DNA. These mutations are associated with the occurrence of hereditary nonpolyposis colorectal cancer (HNPCC) and are a common feature in the progression of many other cancers. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of MLH3 of human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1228-1453 mapping at the C-terminus of MLH3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1228-1453 mapping at the C-terminus of MLH3 of human origin |
| Entrez Gene | 27030 |
|---|
Application
Western blot analysis of human recombinant MLH3 fusion protein.| Application Notes | recommended for detection of MLH3 of human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
