Application
Immunofluorescence staining of normal mouse kidney frozen section showing nuclear staining.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded pediatric renal tumor showing nuclear localization of WT1. Kindly provided by Pamila Ramani.Application
Western blot analysis of WT1 expression in MCF7 whole cell lysate (A) and nuclear extract (B).| Product Name | WT1 Antibody (C-19) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of WT1 of human origin |
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | Wilms’ tumor (WT) is an embryonal malignancy of the kidney that affects 1 in 10,000 infants and, like retinoblastoma, is observed in both sporadic and inherited forms. The Wilms’ tumor locus has been mapped at chromosome 11p13 as a tumor suppressor gene which encodes a DNA binding protein with four zinc fingers and a glutamine-proline rich amino-terminus. The Wilms’ tumor protein (WT1) binds the DNA sequence GCGGGGGCG, a recognition element common to the early growth response (Egr) family of Zn2+ finger transcriptional activators. However, in contrast to Egr transcription factors, WT1 behaves as a transcriptional repressor in transient transfection assays with synthetic promotor constructs. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCWB |
| recommended for detection of WT1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of WT1 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of WT1 of human origin |
| Entrez Gene | 7490 |
|---|
Application
Immunofluorescence staining of normal mouse kidney frozen section showing nuclear staining.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded pediatric renal tumor showing nuclear localization of WT1. Kindly provided by Pamila Ramani.
Application
Western blot analysis of WT1 expression in MCF7 whole cell lysate (A) and nuclear extract (B).| Application Notes | recommended for detection of WT1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
