Application
Immuno?fluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of MCM4 expression in HeLa (A) and K-562 (B) nuclear extracts.| Product Name | MCM4 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDC21,P1-CDC21,DNA replication licensing factor MCM4 |
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM4 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1 to S phase. The MCM proteins each contain an ATP-binding motif, which is predicted to mediate ATP-dependent opening of double-stranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the postreplicative state of the cell. MCM2/MCM4 complexes function as substrates for Cdc2/cyclin B in vitro. Cleavage of MCM3, which can be prevented by caspase inhibitors, results in the inactivation of the MCM complex (composed of at least MCM proteins 2-6) during apoptosis. A complex composed of MCM4, MCM6 and MCM7 has been shown to be involved in DNA helicase activity, and MCM5 is involved in IFN-©-induced Stat1å transcription activation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIPWB |
| WB:1:100~1:200 IP:1:20~1:100 IF:1:20~1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MCM4 |
|---|---|
| Alternative Names | CDC21 P1-CDC21 DNA replication licensing factor MCM4 |
| Molecular Weight(MW) | 97 kDa |
| Tissue Specificity | epitope corresponding to amino acids 1-300 of MCM4 of human origin |
| Entrez Gene | 4173 |
|---|
Application
Immuno?fluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of MCM4 expression in HeLa (A) and K-562 (B) nuclear extracts.| Application Notes | WB:1:100~1:200 IP:1:20~1:100 IF:1:20~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
