Application
Western blot analysis of TNF-R2 p75 expression in MCF7 (A) and SK-BR-3 (B) whole cell lysates.| Product Name | TNF-R2 Antibody (H-202) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Tumor necrosis factor (TNF) is a pleiotropic cytokine whose function is mediated through two distinct cell surface receptors (1). These receptors, designated TNF-R1 and TNF-R2, are expressed on most cell types (1,2). The majority of TNF functions are primarily mediated through TNF-R1, while signaling through TNF-R2 occurs less extensively and is confined to cells of the immune system (1). Both of these proteins belong to the growing TNF and nerve growth factor (NGF) receptor superfamily, which includes FAS, CD30, CD27 and CD40 (3). The members of this superfamily are type I membrane proteins that share sequence homology confined to the extracellular region (2). TNF-R1 shares a motif termed the “death domain” with FAS and three structurally unrelated signaling proteins, TRADD, FADD and RIP (1,3–8). This death domain is required for transduction of the apoptotic signal (9). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of TNF-R2 of mouse, rat and human origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 260-461 mapping at the C-terminus of TNF-R2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 260-461 mapping at the C-terminus of TNF-R2 of human origin |
| Entrez Gene | 7133 |
|---|
Application
Western blot analysis of TNF-R2 p75 expression in MCF7 (A) and SK-BR-3 (B) whole cell lysates.| Application Notes | recommended for detection of TNF-R2 of mouse, rat and human origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
