Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skeletal muscle tissue showing cytoplasmic staining of myocytes.Application
Western blot analysis of VAMP-1/2/3 expression in mouse brain tissue extract.Application
Western blot analysis of VAMP-1 expression in mouse brain extract.| Product Name | VAMP-1/2/3 Antibody (FL-118) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-118 representing full length vesicle-associated membrane protein (VAMP-1) of human origin |
| Modification Notes | Syntaxins were originally thought to be docking proteins, but have more recently been categorized as anchoring proteins that anchor themselves to the cytoplasmic surfaces of cellular membranes. Syntaxins have been shown to bind to various proteins involved in exocytosis, including VAMPs (vesicle-associated membrane proteins), NSF (N-ethylmaleimide-sensitive factor), SNAP 25 (synaptosomal-associated protein of 25 kDa), SNAPs (soluble NSF attachment proteins) and synaptotagmin. VAMPs, also designated synaptobrevins, including VAMP-1 and VAMP-2, and synaptotagmin, a protein that may function as an inhibitor of exocytosis, are vesicular proteins. SNAPs, including a- and g-SNAP, are cytoplasmic proteins that bind to a membrane receptor complex composed of VAMP, SNAP 25 and Syntaxin. SNAPs mediate the membrane binding of NSF, which is essential for membrane fusion reactions. An additional protein designated synaptophysin may regulate exo-cytosis by competing with SNAP 25 and syntaxins for VAMP binding. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of VAMP-1, VAMP-2 and VAMP-3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-118 representing full length vesicle-associated membrane protein (VAMP-1) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-118 representing full length vesicle-associated membrane protein (VAMP-1) of human origin |
| Entrez Gene | 6843 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skeletal muscle tissue showing cytoplasmic staining of myocytes.
Application
Western blot analysis of VAMP-1/2/3 expression in mouse brain tissue extract.
Application
Western blot analysis of VAMP-1 expression in mouse brain extract.| Application Notes | recommended for detection of VAMP-1, VAMP-2 and VAMP-3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
