Application
Western blot analysis of JAB1 expression in rat liver extract.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon tumor (A) and immunofluorescence staining of methanol-fixed KNRK cells (B) showing nuclear localization.| Product Name | JAB1 Antibody (FL-334) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-334 representing full length JAB1 (Jun-activation domain-binding protein 1) of mouse origin |
| Modification Notes | Genes belonging to the Jun and Fos oncogene families encode nuclear proteins that are found to be associated with a number of transcriptional complexes. The c-Jun protein is a major component of the transcription factor AP-1, originally shown to mediate phorbol ester tumor promoter (TPA)-induced expression of responsive genes through the TPA-response element (TRE). The Jun proteins form homo- and heterodimers which bind the TRE, but the Fos proteins are active only as heterodimers with any of the Jun proteins. Fos/Jun heterodimers have a much higher affinity for the TRE than Jun homodimers. Ha-Ras augments c-Jun activity and stimulates phosphorylation of its activation domain. The coactivator of Jun, designated JAB1 (for Jun-activation domain-binding protein), interacts with c-Jun and Jun D, but not with Jun B or v-Jun. This interaction enhances the transactivating ability of Jun proteins by stabilizing their binding to the TRE. The gene encoding JAB1 maps to human chromosome 8q12.3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of JAB1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-334 representing full length JAB1 (Jun-activation domain-binding protein 1) of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-334 representing full length JAB1 (Jun-activation domain-binding protein 1) of mouse origin |
| Entrez Gene | 10987 |
|---|
Application
Western blot analysis of JAB1 expression in rat liver extract.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon tumor (A) and immunofluorescence staining of methanol-fixed KNRK cells (B) showing nuclear localization.| Application Notes | recommended for detection of JAB1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
