Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing nuclear staining of cells in seminiferous ducts.Application
Western blot analysis of PCNA expression in non-transfected: (A) and human PCNA transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PCNA expression in non-transfected 293T: (A), mouse PCNA transfected 293T: (B) and A-431 (C) whole cell lysates.Application
Western blot analysis of PCNA expression in A-431 (A) and NIH/3T3 (B) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing nuclear localization.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human prostate carcinoma showing nuclear localization.| Product Name | PCNA Antibody (FL-261) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The proliferating cell nuclear antigen (PCNA), a protein synthesized in early G1 and S phases of the cell cycle, functions in cell cycle progression, DNA replication and DNA repair. In early S phase, PCNA exhibits granular distribution and is absent from the nucleoli; however, in late S phase, it relocates to the nucleoli. PCNA exists in two basic forms: one involved in ongoing DNA replication, which localizes specifically to the nucleus, and a second, soluble form, not implicated in constant synthesis. Interestingly, the latter form degrades in the presence of organic solvents, rendering it undetectable by histological methods in tissues using organic fixatives, and thus also providing a method of visualizing only the synthesizing form. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of PCNA of mouse, rat, human, insect, zebrafish, Drosophila and Xenopus origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-261 representing full length human PCNA |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-261 representing full length human PCNA |
| Entrez Gene | 5111 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing nuclear staining of cells in seminiferous ducts.
Application
Western blot analysis of PCNA expression in non-transfected: (A) and human PCNA transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PCNA expression in non-transfected 293T: (A), mouse PCNA transfected 293T: (B) and A-431 (C) whole cell lysates.
Application
Western blot analysis of PCNA expression in A-431 (A) and NIH/3T3 (B) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing nuclear localization.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human prostate carcinoma showing nuclear localization.| Application Notes | recommended for detection of PCNA of mouse, rat, human, insect, zebrafish, Drosophila and Xenopus origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
