Application
Antibodies tested include p-53BP1 (38.Ser 25): (A,B,C) and 53BP1 (H-300): (D,E,F).Application
Western blot analysis of 53BP1 expression in Ramos whole cell lysate.Application
Immunofluorescence staining of normal mouse kidney frozen section showing nuclear and cytoplasmic staining.Application
Western blot analysis of 53BP1 expression in Sol8 (A) and Jurkat (B) whole cell lysates.| Product Name | 53BP1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The p53 binding proteins 53BP1 and 53BP2 (Bbp) bind to the central DNA-binding domain of wild type p53, but do not bind mutant p53. The central DNA-binding domain of p53 is required for site-specific DNA binding and is frequently mutated in malignant tumors. Binding of 53BP1 to the L3 loop of p53 and of 53BP2 to the L2 loop of p53 confirms that the loop is dependent on p53 conformation. Site-specific binding also suggests that 53BP1 and 53BP2 are involved in p53-mediated tumor suppression. 53BP1 was isolated from H258 cells and is expressed in Jurkat cells in both the cytoplasm and the nucleus. The N-terminus of 53BP2 is localized to the cytoplasm, while the C-terminus might be localized in the nucleus. 53BP1 promotes cell proliferation by binding to p202, whereas 53BP2 induces cell death by binding to Bcl2 and NFkB p65. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of 53BP1 of mouse, rat and human origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of 53BP1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of 53BP1 of human origin |
| Entrez Gene | 7158 |
|---|
Application
Antibodies tested include p-53BP1 (38.Ser 25): (A,B,C) and 53BP1 (H-300): (D,E,F).
Application
Western blot analysis of 53BP1 expression in Ramos whole cell lysate.
Application
Immunofluorescence staining of normal mouse kidney frozen section showing nuclear and cytoplasmic staining.
Application
Western blot analysis of 53BP1 expression in Sol8 (A) and Jurkat (B) whole cell lysates.| Application Notes | recommended for detection of 53BP1 of mouse, rat and human origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
