Application
Western blot analysis of Nkx-2.5 expression in non-transfected: (A) and human Nkx-2.5 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Nkx-2.5 expression in non-transfected: (A) and human Nkx-2.5 transfected: (B) 293T whole cell lysates.| Product Name | Nkx-2.5 Antibody (H-114) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Nkx-2.5, which is also designated cardiac specific homeobox protein (Csx), is a homeodomain-containing nuclear transcription protein of the Nkx-2 gene family. These transcriptional activators, which include thyroid transcription factor-1 (TTF-1), regulate the expression of tissue specific genes and are required for maintaining the differentiated phenotypes of various lineages. Nkx-2.5 is a homolog to the tinman protein expressed in Drosophila, and is essential for normal cardiovascular development. Expression of Nkx-2.5 during cardiomyogenesis is required for cardiac septation, in which a single atrium and ventricle are separated into four chambers. During embryonic development, Nkx-2.5 is also expressed in the foregut, thyroid, spleen and stomach, while in the adult expression is predominantly restricted to the heart. Mutations that disrupt Nkx-2.5 can result in atrial-septal defects, embryonic lethality and congenital heart disease. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of Nkx-2.5 of mouse, rat and human origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 24-137 mapping near the N-terminus of Nkx-2.5 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 24-137 mapping near the N-terminus of Nkx-2.5 of human origin |
| Entrez Gene | 1482 |
|---|
Application
Western blot analysis of Nkx-2.5 expression in non-transfected: (A) and human Nkx-2.5 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Nkx-2.5 expression in non-transfected: (A) and human Nkx-2.5 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Nkx-2.5 of mouse, rat and human origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
