Application
Western blot analysis of PMM1 expression in non-transfected 293: (A), human PMM1 transfected 293: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of PMM1 expression in HeLa whole cell lysate.| Product Name | PMM1 Antibody (D-19) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | PMM1 (phosphomannomutase 1), also known as Sec53 or PMMH22, is a 262 amino acid protein that localizes to the cytoplasm and belongs to the eukaryotic PMM family. Expressed at high levels in brain, liver, pancreas and heart and present at lower levels in skeletal muscle, PMM1 catalyzes the conversion of alpha-D-mannose 1-phosphate to D-mannose 6-phosphate, an event that is required for a number of crucial mannosyl transfer reactions. The gene encoding PMM1 maps to human chromosome 22, which houses over 500 genes and is the second smallest human chromosome. Mutations in several of the genes that map to chromosome 22 are involved in the development of Phelan-McDermid syndrome, Neurofibromatosis type 2, autism and schizophrenia. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PMM1 of human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of PMM1 of human origin |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of PMM1 of human origin |
| Entrez Gene | 5372 |
|---|
Application
Western blot analysis of PMM1 expression in non-transfected 293: (A), human PMM1 transfected 293: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of PMM1 expression in HeLa whole cell lysate.| Application Notes | recommended for detection of PMM1 of human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
