Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lymphoma showing nuclear staining.Application
Western blot analysis of Bcl-6 expression in Ramos (A) and NAMALWA (B) whole cell lysates.| Product Name | Bcl-6 Antibody (N-3) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Bcl-6, a transcriptional repressor, binds Stat recognition-like DNA elements and influences germinal center development and Th1/Th2 differentiation. Bcl-6 negatively regulates NFkB expression, thereby inhibiting NFkB-mediated cellular functions. HDAC- and silent information regulator (SIR)-2-dependent acet-ylation of Bcl-6 causes downregulation of activity by inhibiting the ability of Bcl-6 to recruit complexes containing histone deacetylases (HDACs). Bcl-6 is frequently deregulated in non-Hodgkin’s B cell lymphomas. The human BCL6 gene has been shown to encode a protein of 706 amino acids. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of Bcl-6 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 3-484 mapping within the N-terminal two-thirds of Bcl-6 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 3-484 mapping within the N-terminal two-thirds of Bcl-6 of human origin |
| Entrez Gene | 604 |
|---|
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lymphoma showing nuclear staining.
Application
Western blot analysis of Bcl-6 expression in Ramos (A) and NAMALWA (B) whole cell lysates.| Application Notes | recommended for detection of Bcl-6 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
