Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of mSin3B expression in non-transfected: (A) and human mSin3B transfected: (B) 293T whole cell lysates.Application
Western blot analysis of mSin3B expression in non-transfected 293T: (A), human mSin3B transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of mSin3B expression in non-transfected: (A) and mouse mSin3B transfected: (B) 293T whole cell lysates.Application
Western blot analysis of mSin3B expression in non-transfected: (A) and mouse mSin3B transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).Application
Western blot analysis of mSin3B expression in HeLa whole cell lysate.| Product Name | mSin3B Antibody (AK-12) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | It is now well established that Myc regulation of cell proliferation and differentiation involves a family of related transcription factors. One such factor, Max, is an obligate heterodimeric partner for Myc and can also form hetero-dimers with at least four related proteins designated Mad 1, Mxi 1 (alternatively designated Mad 2), Mad 3 and Mad 4. Like Mad 1 and Mxi 1, association of Mad 3 and Mad 4 with Max results in transcriptional repression. Both Myc and the Mad proteins have short half-lives and their synthesis is tightly regulated while Max expression is constitutive and relatively stable. Two related mammalian cDNAs have been identified and shown to encode Mad-binding proteins. Both possess sequence homology with the yeast transcription repressor Sin3 including four conserved paired amphipathic helix (PAH) domains. mSin3A and mSin3B specifically interact with the Mad proteins via their second paired amphipathic helix domain (PAH2). It has been suggested that Mad-Max heterodimers repress transcription by tethering mSin3 to DNA as corepressors. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of mSin3B and, to a lesser extent, mSin3A of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 172-228 mapping within the PAH2 region of mSin3B of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 172-228 mapping within the PAH2 region of mSin3B of human origin |
| Entrez Gene | 23309 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of mSin3B expression in non-transfected: (A) and human mSin3B transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of mSin3B expression in non-transfected 293T: (A), human mSin3B transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of mSin3B expression in non-transfected: (A) and mouse mSin3B transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of mSin3B expression in non-transfected: (A) and mouse mSin3B transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).
Application
Western blot analysis of mSin3B expression in HeLa whole cell lysate.| Application Notes | recommended for detection of mSin3B and, to a lesser extent, mSin3A of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
