Application
Western blot analysis of Dio-1 expression in non-transfected: (A) and human Dio-1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Dio-1 expression in non-transfected 293T: (A), human Dio-1 transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of Dio-1 expression in mouse liver tissue extract.Application
Western blot analysis of Dio-1 expression in LADMAC whole cell lysate.Application
Western blot analysis of Dio-1 expression in Jurkat nuclear extract.| Product Name | Dio-1 Antibody (M-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Dio-1 (Death Inducer-Obliterator-1) is a putative transcription factor that contains two zinc finger motifs. Dio-1 translocates to the nucleus, and activates apoptosis during limb development. Programmed cell death, a highly regulated form of apoptosis, plays an important role in determining the amount of tissue, the shape, and the definition of each digit during limb development. Dio-1 expression is upregulated when an apoptotic signal is detected, and subsequently apoptosis is induced. This process is similar to the expression of NF˚B and NGF in response to external signals. Dio-1 expression is suppressed by caspase inhibitors and Bcl-2 expression. This supports the theory that Dio-1 functions in the onset of programmed cell death. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Dio-1 of mouse, rat and human origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 315-614 representing full length Dio-1 of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 315-614 representing full length Dio-1 of mouse origin |
| Entrez Gene | 11083 |
|---|
Application
Western blot analysis of Dio-1 expression in non-transfected: (A) and human Dio-1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Dio-1 expression in non-transfected 293T: (A), human Dio-1 transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of Dio-1 expression in mouse liver tissue extract.
Application
Western blot analysis of Dio-1 expression in LADMAC whole cell lysate.
Application
Western blot analysis of Dio-1 expression in Jurkat nuclear extract.| Application Notes | recommended for detection of Dio-1 of mouse, rat and human origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
