Application
Western blot analysis of NDUFAF1 expression in Caki-1 whole cell lysate.| Product Name | NDUFAF1 Antibody (H-79) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | NDUFAF1 (NADH dehydrogenase (ubiquinone) 1 alpha subcomplex assembly factor 1), also known as CIA30 (Complex I intermediate-associated protein 30, mitochondrial) or CGI-65, is a 327 amino acid mitochondrial protein that is ubiquitously expressed and belongs to the CIA30 family. The largest multiprotein enzyme of the oxidative phosphorylation (OXPHOS) system, NDUFAF1 functions as a chaperone protein that is involved in the assembly of the mitochondrial NADH ubiquinone oxidoreductase complex I. Complex I plays an important role in the transfer of electrons from NADH to the respiratory chain, a process that is essential for cellular respiration. NDUFAF1 is a crucial component in the early assembly of complex I and mutations in its gene can cause mitochondrial disease. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of NDUFAF1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 180-258 mapping within an internal region of NDUFAF1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 180-258 mapping within an internal region of NDUFAF1 of human origin |
| Entrez Gene | 51103 |
|---|
Application
Western blot analysis of NDUFAF1 expression in Caki-1 whole cell lysate.| Application Notes | recommended for detection of NDUFAF1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
