Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing nuclear envelope and cytoplasmic staining of myocytes.Application
Western blot analysis of Lamin A/C expression in WI 38 (A) and Hs68 (B) whole cell lysates.Application
Western blot analysis of Lamin A expression in non-transfected: (A) and human Lamin A transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Lamin A expression in non-transfected: (A) and human Lamin A transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Lamin A/C expression in non-transfected 293T: (A), mouse Lamin A/C transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed C32 cells showing nuclear envelope localization.| Product Name | Lamin A/C Antibody (H-110) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | A unique family of cysteine proteases has been described that differs in sequence, structure and substrate specificity from any previously described protease family. This family, termed Ced-3/ICE, is comprised of ICE, CPP32, ICH-1/Nedd-2, Tx, Mch2, Mch3 (ICE-LAP3 or CMH-1), Mch4 and ICE-LAP6. Ced-3/ICE family members function as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Nuclear lamins are critical to maintaining the integrity of the nuclear envelope and cellular morphology. The nuclear Lamin A is cleaved by Mch2, but not CPP32. Nuclear Lamin B is fragmented as a consequence of apoptosis by an unidentified member of the ICE family. Lamin C is a splice variant of Lamin A, differing only at the carboxy-terminus. Lamins A and C are identical for the first 566 amino acids, with Lamin C differing only in six unique carboxy-terminal amino acids. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Lamin A/C of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 231-340 mapping within an internal region of Lamin A of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 231-340 mapping within an internal region of Lamin A of human origin |
| Entrez Gene | 4000 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing nuclear envelope and cytoplasmic staining of myocytes.
Application
Western blot analysis of Lamin A/C expression in WI 38 (A) and Hs68 (B) whole cell lysates.
Application
Western blot analysis of Lamin A expression in non-transfected: (A) and human Lamin A transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Lamin A expression in non-transfected: (A) and human Lamin A transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Lamin A/C expression in non-transfected 293T: (A), mouse Lamin A/C transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed C32 cells showing nuclear envelope localization.| Application Notes | recommended for detection of Lamin A/C of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
