Application
Western blot analysis of caspase-3 expression in untreated (A) and Staurosporine treated (B) HeLa whole cell lysates. Note cleaved caspase-3 expression in lane B.Application
Western blot analysis of caspase-3 expression in non-transfected 293T: (A), human caspase-3 transfected 293T: (B) and HuT 78 (C) whole cell lysates.Application
Western blot analysis of human recombinant caspase-3 p11.Application
Western blot analysis of caspase-3 expression in non-transfected 293T: (A), human caspase-3 transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of caspase-3 expression in non-transfected: (A) and human caspase-3 transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of methanol-fixed Hut 78 cells showing cytoplasmic localization.Application
Western blot analysis of caspase-3 precursor expression in HuT 78 (A) and CCRF-HSB-2 (B) whole cell lysates.| Product Name | caspase-3 Antibody (H-277) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Caspase-3, also known as apopain, SCA-1, Yama and CPP32, is an aspartate-specific cysteine protease that belongs to the ICE subfamily of caspases. Caspase-3 is expressed in cells as an inactive precursor from which the p17 and p11 subunits of the mature caspase-3 are proteolytically generated during apoptosis. The caspase-3 precursor is first cleaved at Asp175-Ser176 to produce the p11 subunit and the p20 peptide. Subsequently, the p20 peptide is cleaved at Asp28-Ser29 to generate the mature p17 subunit. The active caspase-3 enzyme is a heterodimer composed of two p17 and two p11 subunits. At the onset of apoptosis, caspase-3 proteolytically cleaves PARP at an Asp216-Gly217 bond. During the execution of the apoptotic cascade, activated caspase-3 releases SREBP from the membrane of the ER in a proteolytic reaction that is distinct from their normal sterol-dependent activation. Caspase-3 cleaves and activates SREBPs between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase-3 also cleaves and activates caspase-6, -7 and -9. The human caspase-3 gene encodes a cytoplasmic protein that is highly expressed in lung, spleen, heart, liver, kidney and cells of the immune system. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of caspase-3 p11, p17 and p20 subunits and full length procaspase-3 of mouse, rat and human origin by WB, IP, IF and ELISA partially cross-reactive with other caspases also reactive with additional species, including equine, canine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against amino acids 1-277 representing full length procaspase-3 of human origin |
|---|---|
| Tissue Specificity | raised against amino acids 1-277 representing full length procaspase-3 of human origin |
| Entrez Gene | 836 |
|---|
Application
Western blot analysis of caspase-3 expression in untreated (A) and Staurosporine treated (B) HeLa whole cell lysates. Note cleaved caspase-3 expression in lane B.
Application
Western blot analysis of caspase-3 expression in non-transfected 293T: (A), human caspase-3 transfected 293T: (B) and HuT 78 (C) whole cell lysates.
Application
Western blot analysis of human recombinant caspase-3 p11.
Application
Western blot analysis of caspase-3 expression in non-transfected 293T: (A), human caspase-3 transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of caspase-3 expression in non-transfected: (A) and human caspase-3 transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed Hut 78 cells showing cytoplasmic localization.
Application
Western blot analysis of caspase-3 precursor expression in HuT 78 (A) and CCRF-HSB-2 (B) whole cell lysates.| Application Notes | recommended for detection of caspase-3 p11, p17 and p20 subunits and full length procaspase-3 of mouse, rat and human origin by WB, IP, IF and ELISA partially cross-reactive with other caspases also reactive with additional species, including equine, canine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
