Western blot analysis of NDUFB7 expression in A-431 (A), HL-60 (B), PC-3 (C) and Raji (D) whole cell lysates and rat brain (E) and mouse ovary (F) tissue extracts.
Complex 1 (also known as NADH dehydrogenase) of the electron transport chain (respiratory chain) is an enzymatic complex that catalyzes the transfer of electrons from NADH to ubiquinone. Free energy from the reaction is conserved in the transfer of protons into the intermembrane space to create an electrochemical proton gradient, a driving force for ATP synthesis. Complex 1 is a complicated, multi-protein, L-shaped complex composed of at least 45 different subunits and located in the mitochondrial inner membrane. NDUB7 (NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7), also known as NADH-ubiquinone oxioreductase B18 subunit, complex I-B18 (CI-B18) or cell adhesion protein SQM1, is a 137 amino acid accessory subunit of complex 1. Ubiquitously expressed, NDUFB7 localizes to the mitochondrial inner membrane on the matrix side. NDUFB7 contains a sevenfold repeat of positively-charged residues that may indicate a role in protein-protein interactions.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WBIPIFELISA
recommended for detection of NDUFB7 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine
Species Reactivity
HumanMouseRat
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Alternative Names
epitope corresponding to amino acids 1-137 representing full length NDUFB7 of human origin
Tissue Specificity
epitope corresponding to amino acids 1-137 representing full length NDUFB7 of human origin
Western blot analysis of NDUFB7 expression in A-431 (A), HL-60 (B), PC-3 (C) and Raji (D) whole cell lysates and rat brain (E) and mouse ovary (F) tissue extracts.
Application Notes
recommended for detection of NDUFB7 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine
Additional Information
Form
Liquid
Storage Instructions
For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.