Application
Western blot analysis of Rootletin expression in Y79 nuclear extract.| Product Name | Rootletin Antibody (H-84) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1207-1290 mapping within an internal region of Rootletin of human origin |
| Modification Notes | Rootletin, also known as CROCC (ciliary rootlet coiled-coil protein), is a 2017 amino acid protein that forms centriole-associated fibrous structures and is an essential component of the ciliary rootlet. Localized to basal bodies and centrosomes in ciliated and nonciliated cells, respectively, Rootletin associates with the proximal ends of basal bodies and, in photoreceptors, functions to form elongated polymers between them. Rootletin is required for centrosome cohesion and, through interaction with C-Nap1 (a centrosomal protein present at the ends of the centrioles), can regulate the linkage of centrioles to basal bodies. Rootletin exists as a homopolymer whose association with centrosomes can be regulated via phosphorylation by Nek2 (NIMA-related kinase 2). Two isoforms exist due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Rootletin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1207-1290 mapping within an internal region of Rootletin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1207-1290 mapping within an internal region of Rootletin of human origin |
| Entrez Gene | 9696 |
|---|
Application
Western blot analysis of Rootletin expression in Y79 nuclear extract.| Application Notes | recommended for detection of Rootletin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
