Application
Western blot analysis of RIP3 expression in human small intestine tissue extract.| Product Name | RIP3 Antibody (H-43) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The death domain is a cytoplasmic domain of approximately 80 amino acids that is necessary for the transduction of apoptotic signals and is present in the apoptosis-mediating receptors TNF-R1 and FAS. Other death domain-containing, but otherwise structurally unrelated proteins have been identified on the basis of their ability to associate with the cytoplasmic domains of TNF-R1 or FAS. One of these proteins, the receptor-interacting protein 3 (RIP3), contains an N-terminal kinase domain and shares extensive homology with RIP and RIP2. However, RIP3 contains a unique C-terminal death domain, which promotes apoptosis. RIP3 can be expressed as two splice variants, RIP3∫ and RIP3©, which contain a truncated N-terminal kinase domain and a distinct and shorter C-terminus. Subsequently, expression of these splice variants downregulates RIP3-mediated apoptosis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of RIP3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 138-180 mapping within an internal region of RIP3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 138-180 mapping within an internal region of RIP3 of human origin |
Application
Western blot analysis of RIP3 expression in human small intestine tissue extract.| Application Notes | recommended for detection of RIP3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
