Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of Islets of Langerhans and glandular cells.Application
Western blot analysis of GADD 34 expression in HeLa (A), Hep G2 (B) and A549 (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of GADD 34 expression in U-937 whole cell lysate.Application
Antibodies tested include GADD 34 (D-8): (A,B) and GADD 34 (H-193): (C,D).| Product Name | GADD 34 Antibody (H-193) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 483-674 of GADD 34 of human origin |
| Modification Notes | It is well established that cell cycle progression is subject to arrest at G1 and G2 checkpoints in response to DNA damage, presumably to allow time for DNA repair prior to entry into S and M phase, respectively. The p53 tumor suppressor is required for one such G1 checkpoint and functions to upregulate expression of GADD 45 and the mitotic inhibitory protein p21. GADD 45 has been shown to stimulate DNA excision repair in vitro and to inhibit entry of cells into S phase, and it apparently acts in concert with GADD 153 in inducing growth arrest. A related DNA-damage inducible gene, GADD 34 (also designated MyD116) has been shown to synergize with GADD 45 or GADD 153 in suppressing cell growth. PEG-3 (progression elevated gene-3) shares significant homology with GADD 34 and is inducible by DNA damage. PEG-3 expression has been shown to be elevated in cells displaying a progressed-transformed phenotype. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of GADD 34 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 483-674 of GADD 34 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 483-674 of GADD 34 of human origin |
| Entrez Gene | 23645 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of Islets of Langerhans and glandular cells.
Application
Western blot analysis of GADD 34 expression in HeLa (A), Hep G2 (B) and A549 (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of GADD 34 expression in U-937 whole cell lysate.
Application
Antibodies tested include GADD 34 (D-8): (A,B) and GADD 34 (H-193): (C,D).| Application Notes | recommended for detection of GADD 34 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
