Application
Western blot analysis of NEDD4-L expression in non-transfected: (A) and human NEDD4-L transfected: (B) 293T whole cell lysates.Application
Western blot analysis of NEDD4-L expression in non-transfected: (A) and human NEDD4-L transfected: (B) 293T whole cell lysates.Application
Western blot analysis of NEDD4-L expression in non-transfected: (A) and mouse NEDD4-L transfected: (B) 293T whole cell lysates.Application
Western blot analysis of NEDD4-L expression in non-transfected: (A) and human NEDD4-L transfected: (B) 293T whole cell lysates.Application
Western blot analysis of NEDD4-L expression in Jurkat (A) and Caki-1 (B) whole cell lysates.| Product Name | NEDD4-L Antibody (I-17) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | NEDD4-L (neural precursor cell expressed, developmentally down-regulated 4-like), also known as RSP5, NEDD4-2 or NEDL3, is a 975 amino acid protein that localizes to the cytoplasm and contains four WW domains, one HECT domain and one C2 domain. Expressed ubiquitously with highest expression in pancreas, prostate and kidney, NEDD4-L functions as an E3 ubiquitin-protein ligase that, characteristic of E3 ligase proteins, accepts ubiquitin (in the form of a thioester) from an E2 ubiquitin-conjugating enzyme and transfers that ubiquitin residue to substrates targeted for degradation. Through its ability to ubiquitinate and induce the proteasome-dependent degradation of proteins such as Smad2 and TGF∫ RII, NEDD4-L is thought to inhibit the TGF∫ signaling pathway, thereby regulating the signaling pathways that control cell growth and differentiation. NEDD4-L is expressed as eight isoforms due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of NEDD4-L of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with NEDD4 and NEDD4-1 also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of NEDD4-L of human origin |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of NEDD4-L of human origin |
| Entrez Gene | 23327 |
|---|
Application
Western blot analysis of NEDD4-L expression in non-transfected: (A) and human NEDD4-L transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of NEDD4-L expression in non-transfected: (A) and human NEDD4-L transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of NEDD4-L expression in non-transfected: (A) and mouse NEDD4-L transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of NEDD4-L expression in non-transfected: (A) and human NEDD4-L transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of NEDD4-L expression in Jurkat (A) and Caki-1 (B) whole cell lysates.| Application Notes | recommended for detection of NEDD4-L of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with NEDD4 and NEDD4-1 also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
