Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human parathyroid gland tissue showing membrane staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of CD98 expression in U-937 (A), HeLa (B) and Hep G2 (C) whole cell lysates.Application
Western blot analysis of CD98 expression in U-937 (A) and HL-60 (B) whole cell lysates. Note glycosylated heavy chain.Application
Immunofluorescence staining of methanol-fixed U-937 cells showing membrane localization.Application
Western blot analysis of human recombinant CD98 fusion protein.| Product Name | CD98 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | CD98 (4F2, CD98, MDU1, 4F2HC, 4T2HC, NACAE) is a disulfide-linked heterodimer composed of a glycosylated heavy chain and a non-glycosylated light chain. CD98 is a scaffolding protein that interacts with basolaterally expressed amino acid transporters and ∫1 integrins and can alter amino acid transport and cell adhesion, migration and branching morphogenesis. The heavy chain is a type II integral membrane protein. CD98 is expressed on T cells and is upregulated upon T cell activation. CD98 is also present on monocytes and at lower levels on granulocytes, platelets and lymphocytes. Evidence suggests that CD98 may play a role in the regulation of T cell activation and proliferation. Alternate transcriptional splice variants, encoding different isoforms exist for the human CD98 gene. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of CD98 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 230-529 mapping at the C-terminus of CD98 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 230-529 mapping at the C-terminus of CD98 of human origin |
| Entrez Gene | 6520 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human parathyroid gland tissue showing membrane staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of CD98 expression in U-937 (A), HeLa (B) and Hep G2 (C) whole cell lysates.
Application
Western blot analysis of CD98 expression in U-937 (A) and HL-60 (B) whole cell lysates. Note glycosylated heavy chain.
Application
Immunofluorescence staining of methanol-fixed U-937 cells showing membrane localization.
Application
Western blot analysis of human recombinant CD98 fusion protein.| Application Notes | recommended for detection of CD98 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
