Application
Western blot analysis of Ramp4 expression in non-transfected: (A) and human Ramp4 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Ramp4/4-2 expression in mouse embryo tissue extract (A) and NIH/3T3 (B) and LNCaP (C) whole cell lysates.Application
Western blot analysis of Ramp4-2 expression in non-transfected: (A) and human Ramp4-2 transfected: (B) 293T whole cell lysates.| Product Name | Ramp4/4-2 Antibody (A-18) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Newly synthesized proteins emerging through the endoplasmic reticulum (ER) membrane enter a unique environment for folding and assembly by associated proteins. RAMP4 (ribosome-attached membrane protein 4), also designated SERP1 (stress-associated endoplasmic reticulum protein family member 1) and RAMP4-2 (ribosome-associated membrane protein 4-2), also known as SERP2 (stress-associated endoplasmic reticulum protein family member 2), are single-pass type IV membrane proteins that localize to the ER and belong to the RAMP4 family. RAMP4 and RAMP4-2 may interact with target proteins during translocation into the lumen of the ER and protect unfolded target proteins against degradation during ER stress. After termination of ER stress, RAMP4 and RAMP4-2 are involved in facilitating glycosylation of target proteins. RAMP4 consists of 66 amino acids and is encoded by a gene located on human chromosome 3, whereas RAMP4-2 is encoded by a gene located on human chromosome 13 and is composed of 61 amino acids. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Ramp4-2 and Ramp4 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with other Ramp family members. also reactive with additional species, including canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the N-terminus of Ramp4-2 of human origin |
|---|---|
| Tissue Specificity | epitope mapping near the N-terminus of Ramp4-2 of human origin |
Application
Western blot analysis of Ramp4 expression in non-transfected: (A) and human Ramp4 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Ramp4/4-2 expression in mouse embryo tissue extract (A) and NIH/3T3 (B) and LNCaP (C) whole cell lysates.
Application
Western blot analysis of Ramp4-2 expression in non-transfected: (A) and human Ramp4-2 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Ramp4-2 and Ramp4 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with other Ramp family members. also reactive with additional species, including canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
