Application
Western blot analysis of KAI 1 expression in non-transfected: (A) and human KAI 1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of human recombinant KAI 1 fusion protein.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human prostate tumor showing membrane staining.| Product Name | KAI 1 Antibody (H-173) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 95-267 mapping at the C-terminus of metastasis suppressor protein (KAI 1) of human origin |
| Modification Notes | The transmembrane 4 superfamily (TM4SF) is a family of leukocyte surface glycoproteins that presumably cross the cell membrane four times. These proteins may be involved in transmembrane signal transduction regulation of cell proliferation, differentiation and motility. Members of this family, which include CD9, CD37, CD53, CD63, CD82 and TAPA-1, share significant sequence homology and an extracellular N-glycosylated domain, implicating these proteins as metastasis suppressors. Only three members of this family have been correlated with metastasis: CD9, CD63 and CD82, also known as KAI 1. KAI 1 is evolutionarily conserved and expressed in a broad range of human tissues, but exhibits reduced expression in human cell lines derived from metastatic prostate tumors. It has been suggested that decreased KAI 1 expression may be involved in the malignant progression of prostate and perhaps other cancers. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAChIP |
| recommended for detection of KAI 1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 95-267 mapping at the C-terminus of metastasis suppressor protein (KAI 1) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 95-267 mapping at the C-terminus of metastasis suppressor protein (KAI 1) of human origin |
| Entrez Gene | 3732 |
|---|
Application
Western blot analysis of KAI 1 expression in non-transfected: (A) and human KAI 1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of human recombinant KAI 1 fusion protein.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human prostate tumor showing membrane staining.| Application Notes | recommended for detection of KAI 1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
