Application
Western blot analysis of CD46 expression in non-transfected 293T: (A), human CD46 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing membrane staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed, control HeLa (A) and CD46 siRNA silenced HeLa (B) cells showing diminished membrane staining in the siRNA silenced cells. Cells probed with CD46 (H-294): .Application
Western blot analysis of CD46 expression in Caki-1 (A) and CCRF-CEM (B) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane staining.| Product Name | CD46 Antibody (H-294) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 35-328 representing the extracellular domain of CD46 of human origin |
| Modification Notes | CD46, also called membrane cofactor protein (MCP), is a transmembrane glycoprotein that exists as a non-disulfide-linked dimer. CD46 regulates the complement cascade by inhibiting C3b and C4b deposited on self tissue. CD46 is a cofactor that binds to C3b and C4b, allowing their degradation by a plasma serine protease called factor I. This function resides in the complement control protein repeats (CCPs), with CCP1–4 essential for regulation. CD46 is widely distributed on thymocytes, T cells, B cells, monocytes, granulocytes, NK cells, platelets, endothelial cells, epithelial cells, fibroblasts, placenta and sperm, but not on erythrocytes. It is the major high affinity receptor for measles virus and human herpes virus. Mouse cells ubiquitously express CRRY, which is a functional ortholog of human decay-accelerating factor (DAF; CD55) and membrane cofactor protein (MCP; CD46). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of CD46 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 35-328 representing the extracellular domain of CD46 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 35-328 representing the extracellular domain of CD46 of human origin |
| Entrez Gene | 4179 |
|---|
Application
Western blot analysis of CD46 expression in non-transfected 293T: (A), human CD46 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing membrane staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed, control HeLa (A) and CD46 siRNA silenced HeLa (B) cells showing diminished membrane staining in the siRNA silenced cells. Cells probed with CD46 (H-294): .
Application
Western blot analysis of CD46 expression in Caki-1 (A) and CCRF-CEM (B) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane staining.| Application Notes | recommended for detection of CD46 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
