| Product Name | Dbs Antibody (H-90) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 288-377 mapping within an internal region of Dbs of human origin |
| Modification Notes | The Dbl family act as guanine nucleotide exchange factors (GEFs) specific for Rho guanosine triphosphatases (GTPases) (1,2). They regulate Rho GTPase function by stimulating formation of the active, GTP-bound state. All Dbl family members invariably possess a tandem domain structure, which consists of a Dbl homology (DH) catalytic domain followed by a pleckstrin homology (PH) regulatory domain (1-3). Dbs (for Dbl’s big sister), also known as Ost or MCF2L, differs from Dbl by the addition of an amino terminal extension and a carboxy terminal SH3 domain (4). Unlike Dbl, it also requires the presence of the PH domain for the intrinsic catalytic activity of the DH domain (1-3). The expression of Dbs is high in several tissues, including brain, and low in thymus and spleen (4). Dbs exhibits guanine nucleotide exchange activity for Rho A and Cdc42 to mediate growth deregulation (2,3,5). Dbs activity involves multiple signaling pathways that include activation of the Elk-1, Jun, and NFkB transcription factors and stimulation of transcription from the cyclin D1 promoter (5). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Dbs of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 288-377 mapping within an internal region of Dbs of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 288-377 mapping within an internal region of Dbs of human origin |
| Entrez Gene | 23263 |
|---|
| Application Notes | recommended for detection of Dbs of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
