Application
Western blot analysis of SmcX expression in non-transfected: (A) and human SmcX transfected: (B) 293T whole cell lysates.Application
Western blot analysis of SmcX expression in non-transfected: (A) and human SmcX transfected: (B) 293T whole cell lysates.| Product Name | SmcX Antibody (H-99) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1462-1560 mapping at the C-terminus of SmcX of human origin |
| Modification Notes | SmcX, also known as JARID1C (jumonji, AT rich interactive domain 1C), MRXJ, KDM5C or XE169, is a nuclear protein that contains one ARID domain, one JmjC domain, one JmjN domain and two PHD-type zinc fingers and belongs to the JARID1 histone demethylase family. Expressed ubiquitously with highest expression in brain and skeletal muscle, SmcX functions as a histone demethylase that removes methyl groups from lysine residues on Histone H3, thereby playing a role in the histone code, as well as transcriptional regulation and chromatin remodeling. SmcX binds iron and å-ketoglutarate as cofactors and can recruit histone deacetylases to neuron silencer elements, thus repressing the transcription of neuronal genes. Defects in the gene encoding SmcX are associated with X-linked mental retardation (XLMR), a condition characterized by cognitive impairment and a low IQ. Multiple isoforms of SmcX are expressed due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of SmcX of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1462-1560 mapping at the C-terminus of SmcX of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1462-1560 mapping at the C-terminus of SmcX of human origin |
| Entrez Gene | 8242 |
|---|
Application
Western blot analysis of SmcX expression in non-transfected: (A) and human SmcX transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of SmcX expression in non-transfected: (A) and human SmcX transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of SmcX of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
