Application
Western blot analysis of 17β-HSD6 expression in Hep G2 (A), DU 145 (B) and PC-3 (C) whole cell lysates and mouse prostate tissue extract (D).Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing cytoplasmic staining.Application
Western blot analysis of 17β-HSD6 expression in mouse prostate tissue extract.Application
Western blot analysis of 17β-HSD6 expression in A172 whole cell lysate.| Product Name | 17β-HSD6 Antibody (A-25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against 17β-HSD6 of human origin |
| Modification Notes | 17∫-HSD6 (17∫ hydroxysteroid dehydrogenase type 6), also known as RODH (retinol dehydrogenase), oxidative 3-å-hydroxysteroid dehydrogenase or HSE (3-å-hydroxysteroid epimerase), belongs to the 17∫-HSD family of proteins that regulate the availability of steroids within various tissues throughout the body. 17∫-HSD6 is an NAD-dependent enzyme that is expressed in prostate and liver tissues. Localizing to the lumenal side of the microsome, 17∫-HSD6 plays an important role in androgen and estrogen catabolism. 17∫-HSD6 exhibits oxidoreductase activity, converting 3å-adiol to dihydrotestosterone, and epimerase activity, converting androsterone to epi-androsterone. Via its ability to inactivate androgens and estrogens, 17∫-HSD6 negatively regulates the signaling activity that is mediated by these steroid hormones. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of 17β-HSD6 of mouse, rat, human and canine origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against 17&beta -HSD6 of human origin |
|---|---|
| Tissue Specificity | raised against 17β-HSD6 of human origin |
| Entrez Gene | 8630 |
|---|
Application
Western blot analysis of 17β-HSD6 expression in Hep G2 (A), DU 145 (B) and PC-3 (C) whole cell lysates and mouse prostate tissue extract (D).
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing cytoplasmic staining.
Application
Western blot analysis of 17β-HSD6 expression in mouse prostate tissue extract.
Application
Western blot analysis of 17β-HSD6 expression in A172 whole cell lysate.| Application Notes | recommended for detection of 17β-HSD6 of mouse, rat, human and canine origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
