Application
Western blot analysis of NCOAT expression in SHP-77 whole cell lysate.| Product Name | NCOAT Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of NCOAT of human origin |
| Modification Notes | NCOAT (nuclear cytoplasmic O-GlcNAcase and acetyltransferase), also known as MGEA5 (meningioma-expressed antigen 5), HEXC or MEA5, is a bifunctional enzyme that functions as both a ∫-hexosaminidase and a histone acetyltransferase. Expressed ubiquitously with highest expression in placenta, brain and pancreas, NCOAT functions as a glycosidase that catalyzes the cleavage of O-GlcNAc residues from GlcNAc-modified proteins. In addition, NCOAT acetylates specific residues on Histone H3 and Histone H4, suggesting an important role in the histone code. The enzymatic activity of NCOAT is optimal at a slightly acidic pH of 5.7-7 and NCOAT function is competitively inhibited by free N-acetylglucosamine. Due to alternative splicing events, NCOAT is expressed as three isoforms. Isoform 1 localizes to the cytoplasm, while isoform 3 localizes to the nucleus. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of NCOAT of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of NCOAT of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of NCOAT of human origin |
| Entrez Gene | 10724 |
|---|
Application
Western blot analysis of NCOAT expression in SHP-77 whole cell lysate.| Application Notes | recommended for detection of NCOAT of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
