Application
Western blot analysis of NCAM expression in non-transfected: (A) and human NCAM transfected: (B) 293T whole cell lysates.Application
Western blot analysis of NCAM expression in non-transfected: (A) and human NCAM transfected: (B) 293T whole cell lysates.Application
Western blot analysis of NCAM expression in non-transfected: (A) and mouse NCAM transfected: (B) 293T whole cell lysates.Application
Western blot analysis of NCAM expression in HeLa (A) and IMR-32 (B) whole cell lysates.| Product Name | NCAM Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of NCAM of human origin |
| Modification Notes | Neural cell adhesion molecules (NCAMs) are a family of closely related cell surface glycoproteins involved in cell to cell interactions during growth and thought to play an important role in embryogenesis and development. The expression of these molecules is widespread in all three germ layers during embryogenesis, but is more restrictive in adult tissues. NCAM expression is observed in a variety of human tumors including neuroblastomas, rhabdo-myosarcomas, Wilms’ tumor, Ewing’s sarcoma and some primitive myeloid malignancies. Multiple isoforms of NCAM have been reported in both mouse and human brain tissue. In humans, NCAMs arise from differential splicing and use of alternative polyadenylation sites of a single gene mapping to 11q23. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of NCAM of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of NCAM of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of NCAM of human origin |
| Entrez Gene | 4684 |
|---|
Application
Western blot analysis of NCAM expression in non-transfected: (A) and human NCAM transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of NCAM expression in non-transfected: (A) and human NCAM transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of NCAM expression in non-transfected: (A) and mouse NCAM transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of NCAM expression in HeLa (A) and IMR-32 (B) whole cell lysates.| Application Notes | recommended for detection of NCAM of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
