Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing cytoplasmic staining of glandular cells.WB
Protein expression levels of e,f) F-actin and g,h) MACF1 measured by western blot: Immunoblot plots of e,g) the proteins, and f,h) ratio of the target protein to the internal reference protein (n = 3).| Product Name | MACF1 Antibody (H-146) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | MACF1 (Microtubule-actin cross-linking factor 1) is a 5,327 amino acid protein that is encoded by the human gene MACF1. MACF1 belongs to the plakin or cytolinker family and contains one actin-binding domain, two CH (calponin-homology) domains, two EF-hand domains, one SH3 domain and thirty-seven spectrin repeats. MACF1 is an F-actin-binding protein which may play a role in cross-linking actin to other cytoskeletal proteins and also binds to microtubules. The spectrin repeats, an important feature found in many proteins involved in cytoskeletal structure, forms a three helix bundle with the second helix (with proline interrupts in some sequences). MACF1 is a cytoplasmic protein expressed mainly in lung, brain, spinal cord, skeletal and cardiac muscle, and skin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCIPWB |
| recommended for detection of MACF1, isoforms 1/2/3/5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 465-610 mapping near the N-terminus of MACF1 isoform 1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 465-610 mapping near the N-terminus of MACF1, isoform 1 of human origin |
| Entrez Gene | 23499 |
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Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing cytoplasmic staining of glandular cells.
WB
Protein expression levels of e,f) F-actin and g,h) MACF1 measured by western blot: Immunoblot plots of e,g) the proteins, and f,h) ratio of the target protein to the internal reference protein (n = 3).| Application Notes | recommended for detection of MACF1, isoforms 1/2/3/5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
