Application
Western blot analysis of Twinfilin-2 expression in non-transfected 293T: (A), human Twinfilin-2 transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.Application
Western blot analysis of Twinfilin-2 expression in non-transfected 293T: (A), mouse Twinfilin-2 transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.Application
Western blot analysis of Twinfilin-2 expression in HeLa whole cell lysate.| Product Name | Twinfilin-2 Antibody (T-14) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within an internal region of Twinfilin-2 of human origin |
| Modification Notes | Twinfilin-2, also known as TWF2, A6r, A6RP or PTK9L, is a 349 amino acid protein that localizes to the perinuclear region of the cytoplasm, as well as to the cytoskeleton, and contains two ADF-H domains. Expressed ubiquitously, Twinfilin-2 functions as an Actin-binding protein that is able to both bind and sequester G-Actin and cap the barbed ends of Actin filaments, thereby inhibiting Actin polymerization and playing a role in Actin-related motile and morphological processes. Twinfilin-2 is subject to post-translational phosphorylation by PKC zeta. The gene encoding Twinfilin-2 maps to human chromosome 3, which houses over 1,100 genes, including a chemokine receptor (CKR) gene cluster and a variety of human cancer-related gene loci. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Twinfilin-2 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with Twinfilin-1 also reactive with additional species, including canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of Twinfilin-2 of human origin |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of Twinfilin-2 of human origin |
| Entrez Gene | 11344 |
|---|
Application
Western blot analysis of Twinfilin-2 expression in non-transfected 293T: (A), human Twinfilin-2 transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.
Application
Western blot analysis of Twinfilin-2 expression in non-transfected 293T: (A), mouse Twinfilin-2 transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.
Application
Western blot analysis of Twinfilin-2 expression in HeLa whole cell lysate.| Application Notes | recommended for detection of Twinfilin-2 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with Twinfilin-1 also reactive with additional species, including canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
