Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung carcinoma tissue showing cytoplasmic localization.Application
Western blot analysis of eIF4B phosphorylation expression in untreated (A) and EGF treated (B) HeLa whole cell lysates.| Product Name | p-eIF4B Antibody (Ser 422) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Ser 422 phosphorylated eIF4B of m and h origin by WB, IP, IF and IHC(P) |
| Modification | Phosphoralated |
| Modification Notes | The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. These interactions are facilitated, in part, by the eukaryotic initiation factor 4 family (eIF4) of proteins that are involved in the early initiation of protein synthesis. eIF4B (eukaryotic translation initiation factor 4B) is a 611 amino acid protein that contains one RNA recognition motif and belongs to the eIF4 protein family. Required for proper mRNA/ribosome binding, eIF4B associates with other eIF4 proteins, such as eIF4A, and promotes the ATP-dependent unwinding activity of select eukaryotic initiation factors. The gene encoding eIF4B maps to human chromosome 12, which encodes over 1,100 genes and comprises approximately 4.5% of the human genome. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Ser 422 phosphorylated eIF4B of m and h origin by WB IP IF and IHC(P) |
|---|---|
| Tissue Specificity | recommended for detection of Ser 422 phosphorylated eIF4B of m and h origin by WB, IP, IF and IHC(P) |
| Entrez Gene | 1975 |
|---|
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung carcinoma tissue showing cytoplasmic localization.
Application
Western blot analysis of eIF4B phosphorylation expression in untreated (A) and EGF treated (B) HeLa whole cell lysates.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
