Application
Western blot analysis of MACF1-4 expression in human lung tissue extract.| Product Name | MACF1-4 Antibody (H-220) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1561-1780 mapping within an internal region of MACF1, isoform 4 of human origin |
| Modification Notes | MACF1-4 (Microtubule-Actin cross-linking factor 1, isoform 4) is a 5,327 amino acid protein that is encoded by the human gene MACF1. MACF1-4 belongs to the plakin or cytolinker family and contains one Actin-binding domain, two CH (calponin-homology) domains, two EF-hand domains, nineteen plectin repeats and thirty-seven spectrin repeats. MACF1-4 is an F-Actin-binding protein which may play a role in cross-linking cytoskeletal proteins by binding intermediate filaments to the N-terminal plectin repeats and microtubules to the C-terminus. The spectrin repeats, an important feature found in many proteins involved in cytoskeletal structure, forms a three helix bundle with the second helix (with proline interrupts in some sequences). MACF1-4 is a cytoplasmic protein ex-pressed mainly in heart, lung, pituitary and placenta; however, it is not found in brain, kidney, liver, pancreas or skeletal muscle. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of MACF1, isoform 4 of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1561-1780 mapping within an internal region of MACF1 isoform 4 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1561-1780 mapping within an internal region of MACF1, isoform 4 of human origin |
| Entrez Gene | 23499 |
|---|
Application
Western blot analysis of MACF1-4 expression in human lung tissue extract.| Application Notes | recommended for detection of MACF1, isoform 4 of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
